Plasmid

Part:BBa_K4706018:Design

Designed by: Niklas Küppers   Group: iGEM23_Duesseldorf   (2023-10-01)


pSB1C30YIpHR-HO Gal1 induced RFP A2 AtBAG6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3049
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3049
    Illegal BglII site found at 2500
    Illegal BglII site found at 3899
    Illegal BglII site found at 4247
    Illegal XhoI site found at 1533
    Illegal XhoI site found at 2425
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3049
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3049
    Illegal XbaI site found at 3064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 3220
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pSB1C30YIpHR-HO Gal1 induced RFP A2 AtBAG6


Source

pSB1C30YIpHR-HO Gal1 induced RFP A2 AtBAG6

References